Antigen

Antigen Class

TFs and others

Antigen

Epitope tags

Cell type

Cell type Class

Breast

Cell type

T-47D

Primary Tissue

Breast

Tissue Diagnosis

Adenocarcinoma Ductal

Sample

source_name

T47D_ESR1PCDH11X_noE2_HA_ChIPseq

cell line

T47D

cell subtype

Stable ESR1-PCDH11X fusion expressing model

condition

Hormone-deprived media

chip antibody

HA (Santa Cruz, sc-7392)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Cells were grown hormone-deprived media for 7 days before sonicated chromatin extracts were subjected to HA-ChIP, followed by crosslink reversal and chromatin isolation Wafergen PrepX DNA ChIP library preparation protocol was used.

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

33976931

Reads aligned (%)

83.5

Duplicates removed (%)

1.8

Number of peaks

2162 (qval < 1E-05)

hg19

Number of total reads

33976931

Reads aligned (%)

82.7

Duplicates removed (%)

3.2

Number of peaks

2007 (qval < 1E-05)