Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Epitope tags

Cell type

Cell type Class
Breast
Cell type
T-47D
Primary Tissue
Breast
Tissue Diagnosis
Adenocarcinoma Ductal

Attributes by original data submitter

Sample

source_name
T47D_ESR1YAP1_noE2_HA_ChIPseq
cell line
T47D
cell subtype
Stable ESR1-YAP1 fusion expressing model
condition
Hormone-deprived media
chip antibody
HA (Santa Cruz, sc-7392)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were grown hormone-deprived media for 7 days before sonicated chromatin extracts were subjected to HA-ChIP, followed by crosslink reversal and chromatin isolation Wafergen PrepX DNA ChIP library preparation protocol was used.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg38

Number of total reads
30452122
Reads aligned (%)
89.1
Duplicates removed (%)
1.5
Number of peaks
1010 (qval < 1E-05)

hg19

Number of total reads
30452122
Reads aligned (%)
88.2
Duplicates removed (%)
2.9
Number of peaks
1078 (qval < 1E-05)

Base call quality data from DBCLS SRA