Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Blood
Cell type
NALM-6
Primary Tissue
Blood
Tissue Diagnosis
Leukemia

Attributes by original data submitter

Sample

source_name
B cell precursor leukemia
cell line
Nalm6
cell type
B cell precursor leukemia cell line
chip antibody
none

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
A frozen cell pellet containing 10 million cells was sent to Active Motif for ChIP and library preparation. The sample was divided into an aliquot for ChIP using an antibody to H3K27Ac (Active Motif AM, cat# 39133, lot# 8) and an input control. Single-end sequencing was performed using an Illumina NextSeq 500 generating 76 cycles for each sequencing read.

Sequencing Platform

instrument_model
NextSeq 500

hg38

Number of total reads
121345892
Reads aligned (%)
95.1
Duplicates removed (%)
12.9
Number of peaks
2990 (qval < 1E-05)

hg19

Number of total reads
121345892
Reads aligned (%)
94.3
Duplicates removed (%)
14.5
Number of peaks
2096 (qval < 1E-05)

Base call quality data from DBCLS SRA