Antigen

Antigen Class

RNA polymerase

Antigen

RNA polymerase II

Cell type

Cell type Class

Blood

Cell type

Lymphoma, Mantle-Cell

MeSH Description

A form of non-Hodgkin lymphoma having a usually diffuse pattern with both small and medium lymphocytes and small cleaved cells. It accounts for about 5% of adult non-Hodgkin lymphomas in the United States and Europe. The majority of mantle-cell lymphomas are associated with a t(11;14) translocation resulting in overexpression of the CYCLIN D1 gene (GENES, BCL-1).

Sample

source_name

Mantle cell lymphoma

chip antibody

POL2 (Fisher scientific NC0888291)

treatment

50nM THZ1

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Samples were homogenized with rotor-stator device. Lysates were sonicated via Bioruptor. Protein-DNA complexes were isolated via immunoprecipitation and prepared for sequencing with Rubicon Thruplex DNA-seq kit.

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

36521350

Reads aligned (%)

74.6

Duplicates removed (%)

24.1

Number of peaks

20018 (qval < 1E-05)

hg19

Number of total reads

36521350

Reads aligned (%)

74.0

Duplicates removed (%)

24.2

Number of peaks

19853 (qval < 1E-05)