Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K27ac

Cell type

Cell type Class
Blood
Cell type
Lymphoma, Mantle-Cell
MeSH Description
A form of non-Hodgkin lymphoma having a usually diffuse pattern with both small and medium lymphocytes and small cleaved cells. It accounts for about 5% of adult non-Hodgkin lymphomas in the United States and Europe. The majority of mantle-cell lymphomas are associated with a t(11;14) translocation resulting in overexpression of the CYCLIN D1 gene (GENES, BCL-1).

Attributes by original data submitter

Sample

source_name
Mantle cell lymphoma
chip antibody
H3K27AC (Abcam ab4729)
treatment
none

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Samples were homogenized with rotor-stator device. Lysates were sonicated via Bioruptor. Protein-DNA complexes were isolated via immunoprecipitation and prepared for sequencing with Rubicon Thruplex DNA-seq kit.

Sequencing Platform

instrument_model
NextSeq 500

hg38

Number of total reads
27627617
Reads aligned (%)
92.9
Duplicates removed (%)
11.1
Number of peaks
37028 (qval < 1E-05)

hg19

Number of total reads
27627617
Reads aligned (%)
92.7
Duplicates removed (%)
11.4
Number of peaks
36891 (qval < 1E-05)

Base call quality data from DBCLS SRA