matrix-depositing MC3T3-E1 subclone 4 cells at day 9
cell type
matrix-depositing osteoblast
passages
28-31
strain
C57BL/6
chip antibody
Runx2 antibody (M70, Santa Cruz Biotechnologies, Inc.)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were obtained from sonicated nuclei and Runx2-DNA complexes were isolated with Runx2 antibody (M70). Libraries of purified DNA were generated using Illumina SR adapters (Illumina) following manufacturer’s manual, and were selected for the inserted fragments of 200 ± 50 bp, and sequenced 36 bases on an Illumina Genome Analyzer II.