Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
CTBP1

Cell type

Cell type Class
Gonad
Cell type
SK-OV-3
Primary Tissue
Ovary
Tissue Diagnosis
Adenocarcinoma

Attributes by original data submitter

Sample

source_name
Ovarian cancer
cell line
SKOV3
antibody
anti-CtBP
treatment
8uM Fulverstrant for 24 hours

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
The cells were crosslined by 1% Formaldehyde for 10 minites. After the crosslink was quenched by 125mM Glycine. Then the chromatin was fragmented by sonication. The chromatin was subjected pull down by specific antibodies respectively as indicated. The DNA was eventually extracted after the de-crosslinking and Phenol-choloroform extraction. ChIP-seq library was constructed according the the protocol provided by the NEBNext® ChIP-Seq Library Prep Master Mix Set for Illumina® kit.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg19

Number of total reads
12036459
Reads aligned (%)
83.4
Duplicates removed (%)
4.1
Number of peaks
6554 (qval < 1E-05)

hg38

Number of total reads
12036459
Reads aligned (%)
84.6
Duplicates removed (%)
3.9
Number of peaks
6637 (qval < 1E-05)

Base call quality data from DBCLS SRA