Antigen

Antigen Class

Histone

Antigen

H3K4me3

Cell type

Cell type Class

Digestive tract

Cell type

KYSE-150

Primary Tissue

Esophagus

Tissue Diagnosis

Carcinoma Squamous Cell

Sample

source_name

KYSE150_shCtrl_H3K4me3

cell line

KYSE150

treatment

shCtrl

chip antibody

H3K4me3

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Chromatin immunoprecipitation (ChIP) was performed using formaldehyde crosslinked chromatin sonicated to an average size of 200-600 bp and the indicated antibodies. 5-10 ng of DNA was processed using NEBNext and Illumina ChIP seq kits according to the manufacturer's instructions. In brief, DNA was adaptor-ligated, subjected to gel size selection, amplified and analyzed by Solexa/Illumina high-throughput sequencing.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

57474012

Reads aligned (%)

98.4

Duplicates removed (%)

40.8

Number of peaks

31976 (qval < 1E-05)

hg19

Number of total reads

57474012

Reads aligned (%)

98.2

Duplicates removed (%)

41.1

Number of peaks

32637 (qval < 1E-05)