Antigen

Antigen Class

TFs and others

Antigen

KDM4C

Cell type

Cell type Class

Digestive tract

Cell type

KYSE-150

Primary Tissue

Esophagus

Tissue Diagnosis

Carcinoma Squamous Cell

Sample

source_name

KYSE150_shCtrl_JMJD2C

cell line

KYSE150

treatment

shCtrl

chip antibody

JMJD2C

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Chromatin immunoprecipitation (ChIP) was performed using formaldehyde crosslinked chromatin sonicated to an average size of 200-600 bp and the indicated antibodies. 5-10 ng of DNA was processed using NEBNext and Illumina ChIP seq kits according to the manufacturer's instructions. In brief, DNA was adaptor-ligated, subjected to gel size selection, amplified and analyzed by Solexa/Illumina high-throughput sequencing.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg19

Number of total reads

23814104

Reads aligned (%)

95.5

Duplicates removed (%)

37.6

Number of peaks

15987 (qval < 1E-05)

hg38

Number of total reads

23814104

Reads aligned (%)

95.8

Duplicates removed (%)

37.1

Number of peaks

15664 (qval < 1E-05)