GSM3204310: 16 hr TNF-a MLL4 ChIP-seq; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
KMT2D
Cell type
Cell type Class
Digestive tract
Cell type
SW 480
Primary Tissue
unresolved
Tissue Diagnosis
unresolved
Attributes by original data submitter
Sample
source_name
SW480 Cells
cell line
SW480
cell type
Colon Cancer Cells
genotype/variation
control
treatment
TNF-a
chip antibody
anti-MLL4 provided by Kai Ge (NIH)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
For ChIP, cells were crosslinked, lysates from sonicated nuclei were prepared, and protein-DNA complexes were immunoprecipitated with corresponding antibodies. Please refer to the methods section for detailed protocols. ChIP-seq libraries were prepared according to Illumina's ChIP Truseq protocol. Briefly, ChIP DNA was end-repaired and adaptors were ligated to the ends of the DNA fragments. Adaptor-ligated ChIP DNA fragments with average size of 350 bp were used to construct libraries and single-end sequenced on Illumina HiSeq 4000.