For ChIP-Seq: 10min formaldehyde fixation followed by sonication. Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with SUMO antibody. Libraries prepared using Microplex library Preparation kit v2 (C05010014, Diagenode) following manufacturer's protocol (V2 02.15). Libraries were size-selected (200-400bp) and clenaed-up using Agencourt AMPure XP beads (#A63881, Beckman).