GSM3191756: ChIP-seq INPUT ES siCtrl rep1; Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Pluripotent stem cell
Cell type
ES cells
NA
NA
Attributes by original data submitter
Sample
source_name
mouse embryonic stem cells
cell type
mouse embryonic stem (ES) cells
genotype/variation
siCtrl
passages
15-20
chip antibody
None
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
For ChIP-Seq: 10min formaldehyde fixation followed by sonication (histone marks) or Mnase digestion (transcription factors). Immunoprecipitation was performed using ChIP-IT kit (#53040, Active Motif) following manufacturer's protocol. Experiments were done in duplicates. For ChIP-Seq: Libraries prepared using Microplex library Preparation kit v2 (C05010014, Diagenode) following manufacturer's protocol (V2 02.15). Libraries were size-selected (200-400bp) and clenaed-up using Agencourt AMPure XP beads (#A63881, Beckman). For ATAC-seq: according to Buenrostro et al., Nature Methods, 2013. 30min treatment with Tn5 nuclease on isolated nuclei. Experiments were done in triplicates. For ATAC-seq: Elute transposed DNA was PCR-amplified using barcode primers and purified using PCR Cleanup kit (QIAGEN)