Human HCT116 cells were grown to 200,000 cells per well, crosslinked, and sheared using 96-well plate PIXUL sonicator. Fragmented chromatin was used in ChIP assays with the listed antibodies. ChIP and library prep was performed for each factor from a single well using Active Motif's Low Cell ChIP-Seq Kit (Catalog number 53084). Libraries were sequenced on a NextSeq 500 as PE75 or SE75 with dual 8bp indexing, allowing for PCR de-duplication with molecular identifiers at the i5 position (see product manual).