Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Gonad

Cell type

Granulosa Cells

MeSH Description

Supporting cells for the developing female gamete in the OVARY. They are derived from the coelomic epithelial cells of the gonadal ridge. Granulosa cells form a single layer around the OOCYTE in the primordial ovarian follicle and advance to form a multilayered cumulus oophorus surrounding the OVUM in the Graafian follicle. The major functions of granulosa cells include the production of steroids and LH receptors (RECEPTORS, LH).

Sample

source_name

granulosa cells

cell type

peri-ovulatory granulosa cells

strain

CBA x C57BL/6 F1

age

21 days

antibody

none

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and protein-DNA complexes were isolated with antibody. Chromatin was purified by phenol-chloroform extraction and ethanol precipitation. Illumina sequencing libraries were prepared from the ChIP and input DNA by the standard consecutive enzymatic steps of end-polishing, dA-addition, and adaptor ligation. After a final PCR amplification step, the resulting DNA libraries were quantified and sequenced on Illumina's NextSeq 500 (75 nt reads, single end).

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

45504914

Reads aligned (%)

97.6

Duplicates removed (%)

8.8

Number of peaks

698 (qval < 1E-05)

mm9

Number of total reads

45504914

Reads aligned (%)

97.5

Duplicates removed (%)

8.8

Number of peaks

705 (qval < 1E-05)