Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K4me3

Cell type

Cell type Class
Adult
Cell type
Sperm
NA
NA

Attributes by original data submitter

Sample

source_name
Mature sperm isolated from adult males
strain and genotype
CB1489 him-8(e1489)
purity of sample
~99%
chip antibody
H3K4me3 (Wako, MABI0304, #305-34819)
xchromosome
0.5:1 (50% of sperm have an X, another 50% do not have an X)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Sperm chromatin was crosslinked in 1% formaldehyde for 52 sec, digested with micrococcal nuclease, and sonicated with Covaris. Libraries were prepared using MicroPlex Library Preparation kit v2 (Diagenode). Size selection was performed after library amplification using AMPure XP beads to enrich for 100-300 bp inserts.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

ce11

Number of total reads
29014519
Reads aligned (%)
76.6
Duplicates removed (%)
89.4
Number of peaks
3682 (qval < 1E-05)

ce10

Number of total reads
29014519
Reads aligned (%)
76.6
Duplicates removed (%)
89.4
Number of peaks
3683 (qval < 1E-05)

Base call quality data from DBCLS SRA