0.5:1 (50% of sperm have an X, another 50% do not have an X)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Sperm chromatin was crosslinked in 1% formaldehyde for 52 sec, digested with micrococcal nuclease, and sonicated with Covaris. Libraries were prepared using MicroPlex Library Preparation kit v2 (Diagenode). Size selection was performed after library amplification using AMPure XP beads to enrich for 100-300 bp inserts.