GSM3184658: FOS TAM ChIPseq; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
FOS
Cell type
Cell type Class
Breast
Cell type
MCF 10A
Primary Tissue
Breast
Tissue Diagnosis
Fibrocystic Disease
Attributes by original data submitter
Sample
source_name
MCF10A-ER-Src cell
tissue
Breast
cell line
MCF10A-ER-Src
chip target
FOS
transformation status
transformed
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
The ChIP experiments follow the ENCODE protocol, http://genome.ucsc.edu/ENCODE/, using the following antibodies: STAT3 (Cell Signaling, 9131), NFKB1 (Sigma, HPA027305), FOS (Santa Cruz Biotech, SC-7202x), JUN (Santa Cruz Biotech, SC-1694) and JUNB (Santa Cruz Biotech, SC-73). We performed the ChIP experiments, using the non-transformed MCF10A-ER-Src cells and the cells after 12hr of cell transformation. Library preparation and sequencing were carried out as per ENCODE protocols, and a detailed protocol is available at http://genome.ucsc.edu/ENCODE/