Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Embryo

Cell type

Hindlimb

MeSH Description

Either of two extremities of four-footed non-primate land animals. It usually consists of a FEMUR; TIBIA; and FIBULA; tarsals; METATARSALS; and TOES. (From Storer et al., General Zoology, 6th ed, p73)

Sample

source_name

Hindlimb

strain

B6CBAF1/J

age

E12.5

tissues

Proximal hindlimb

genotype

HoxDdel(8-13)

antibody

none

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Chromatin immunoprecipitated experiment was performed as previously described (Beccari et al. 2016). Micro-dissected limb tissues from mouse and chick embryos were cross-linked with 1% formaldehyde/PBS for 15 min at room temperature. Chromatin was sheared and used for each immunoprecipitation with anti-H3K27ac (ab4729, Abcam), anti-H3K27me3 (07-449, Merck Millipore), anti-H3K4me1 (ab8895, Abcam) and anti-CTCF (61311, Active Motif), respectively. Libraries were prepared with at least 5 ng of purified DNA following Illumina TruSeq ChIP library preparation guide. Sequencing was performed with 100-bp single-end.

Sequencing Platform

instrument_model

Illumina HiSeq 4000

mm10

Number of total reads

19184449

Reads aligned (%)

95.5

Duplicates removed (%)

22.4

Number of peaks

380 (qval < 1E-05)

mm9

Number of total reads

19184449

Reads aligned (%)

95.4

Duplicates removed (%)

22.5

Number of peaks

341 (qval < 1E-05)