Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
SMAD3

Cell type

Cell type Class
Cardiovascular
Cell type
HCASMC
NA
NA

Attributes by original data submitter

Sample

source_name
HCASMC
cell type
Human coronary artery smooth muscle cells (HCASMC)
chip antibody
SMAD3

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
HCASMC were crosslinked in the dish, 2 million cells, and harvested. ChIP was performed using the SMAD3 antibody Abcam, ab28379, and library was prepared using standard procedures. Briefly, DNA was prepared for end repair (Lucigen Endi-it, ER0720) and “A” tailing (NEB Klenow, M0212S), adaptor ligation (Promega, M180A), and library amplification (NEB, M0531S). ChIP-seq libraries were sequenced on HiSeq X10 for 150-bp paired-end sequencing.

Sequencing Platform

instrument_model
HiSeq X Ten

hg19

Number of total reads
26900009
Reads aligned (%)
55.1
Duplicates removed (%)
18.4
Number of peaks
448 (qval < 1E-05)

hg38

Number of total reads
26900009
Reads aligned (%)
55.7
Duplicates removed (%)
18.2
Number of peaks
469 (qval < 1E-05)

Base call quality data from DBCLS SRA