GSM3172725: David TC71 SMC1 1; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
SMC1A
Cell type
Cell type Class
Bone
Cell type
TC-71
Primary Tissue
Bone
Tissue Diagnosis
Sarcoma Ewings
Attributes by original data submitter
Sample
source_name
TC-71 cells
antibody
SMC1
cell line
TC-71 cells
phase_of_therapy
post-chemotherapy
cell type
Ewing sarcoma cells
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were crosslinked with formaldehyde and lysed to obtain purified nuclei that were sonicated to shear chromatin. Protein-DNA complexes were then isolated using Dynabeads with corresponding antibodies, and DNA was isolated through reverse crosslinking and purified by phenol chloroform extraction in order to be used for library construction. Libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit from NEB (E7645), according to the manufacturer's protocol.