GSM3172718: David TC32 SMC1 2; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
TFs and others
Cell type Class
Attributes by original data submitter
Ewing sarcoma cells
Sequenced DNA Library
Cells were crosslinked with formaldehyde and lysed to obtain purified nuclei that were sonicated to shear chromatin. Protein-DNA complexes were then isolated using Dynabeads with corresponding antibodies, and DNA was isolated through reverse crosslinking and purified by phenol chloroform extraction in order to be used for library construction. Libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit from NEB (E7645), according to the manufacturer's protocol.