GSM3172706: Kelly MYCN CS 2; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
MYCN
Cell type
Cell type Class
Neural
Cell type
KELLY
Primary Tissue
Brain
Tissue Diagnosis
Neuroblastoma
Attributes by original data submitter
Sample
source_name
Kelly cells
antibody
MYCN
tae response
sensitive
treatment
DMSO
cell line
Neuroblastoma Kelly cells
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were crosslinked with formaldehyde and lysed to obtain purified nuclei that were sonicated to shear chromatin. Protein-DNA complexes were then isolated using Dynabeads with corresponding antibodies, and DNA was isolated through reverse crosslinking and purified by phenol chloroform extraction in order to be used for library construction. Libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit from NEB (E7645), according to the manufacturer's protocol.