Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Blood
Cell type
Acute myeloid leukemia
NA
NA

Attributes by original data submitter

Sample

source_name
acute myeloid leukemia
cell line
NOMO-1 expressing FLAG_ZFP64
treatment
Stable cell line was generated by infecting NOMO-1 with FLAG-ZFP64 lentivirus
chip antibody
none

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cell lysates were clarified from sonicated nulcei and ZFP64-DNA or histone-DNA complexs were isolated with ZFP64 or histone antibody. Libraries were prepared using Illumina TruSeq ChIP Sample Prep kit following manufacture's protocol. Briefly, ChIP DNA was end-repaired, 3'-adenylated, and then ligated with indexed adaptor. These DNA was size selected (200-400 bp) via agarose gel electrophoresis, and PCR amplified. Final products were purified using AMPure XP beads.

Sequencing Platform

instrument_model
NextSeq 500

hg38

Number of total reads
211804448
Reads aligned (%)
96.9
Duplicates removed (%)
7.2
Number of peaks
4248 (qval < 1E-05)

hg19

Number of total reads
211804448
Reads aligned (%)
96.1
Duplicates removed (%)
8.8
Number of peaks
2134 (qval < 1E-05)

Base call quality data from DBCLS SRA