Antigen

Antigen Class

TFs and others

Antigen

ZFP64

Cell type

Cell type Class

Blood

Cell type

MOLM-13

Primary Tissue

Blood

Tissue Diagnosis

Leukemia Acute Myelogenous

Sample

source_name

acute myeloid leukemia

cell line

MOLM-13

chip antibody

ZFP64(Proteintech Group,17187-1-AP)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Cell lysates were clarified from sonicated nulcei and ZFP64-DNA or histone-DNA complexs were isolated with ZFP64 or histone antibody. Libraries were prepared using Illumina TruSeq ChIP Sample Prep kit following manufacture's protocol. Briefly, ChIP DNA was end-repaired, 3'-adenylated, and then ligated with indexed adaptor. These DNA was size selected (200-400 bp) via agarose gel electrophoresis, and PCR amplified. Final products were purified using AMPure XP beads.

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

15230006

Reads aligned (%)

78.2

Duplicates removed (%)

43.9

Number of peaks

14271 (qval < 1E-05)

hg19

Number of total reads

15230006

Reads aligned (%)

77.8

Duplicates removed (%)

44.5

Number of peaks

14286 (qval < 1E-05)