Antigen

Antigen Class

TFs and others

Antigen

Men1

Cell type

Cell type Class

Blood

Cell type

Th2 Cells

MeSH Description

A subset of helper-inducer T-lymphocytes which synthesize and secrete the INTERLEUKINS IL-4; IL-5; IL-6; and IL-10. These cytokines influence B-cell development and antibody production as well as augmenting humoral responses.

Sample

source_name

KJ1+ naive CD4 T cells

strain background

BALB/c

genotype/variation

DO11.10 OVA-specific TCR transgenic

tissue

spleen

cell type

KJ1+ naive CD4 T cells

chip antibody

Menin

chip antibody vendor

Bethyl Laboratories

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and transcription factor- or histone-DNA complexes were isolated with antibody. Libraries were prepared according to Illumina's instructions accompanying the ChIP Sample Prep Kit

Sequencing Platform

instrument_model

Illumina Genome Analyzer IIx

mm10

Number of total reads

45936113

Reads aligned (%)

82.2

Duplicates removed (%)

23.2

Number of peaks

14443 (qval < 1E-05)

mm9

Number of total reads

45936113

Reads aligned (%)

82.0

Duplicates removed (%)

23.2

Number of peaks

14485 (qval < 1E-05)