Fixed cells were lysed and histone-DNA complexes were isolated with antibodies. Immunostaining half of the protocol includes cell dispensing, fixation, permeabilization, blocking, reaction to primary anti-body and the ChILT probe. The reactive probe are integrated into a proximal genomic region to where the primary antibody is bound upon Tn5 activation. The integrated ChILT DNA is used for the linear amplification of genomic sequences and the transcribed RNAs are utilized for library preparation for deep-sequencing. Fixed cells were lysed and histone-DNA complexes were isolated with antibodies. Libraries were prepared on the Illumina HiSeq1500 and 2500 platforms.