Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Muscle
Cell type
C2C12
Primary Tissue
Skeletal Muscle
Tissue Diagnosis
NOS

Attributes by original data submitter

Sample

source_name
Immortal cell line
cell line
C2C12
antibody target
None
primary antibody
None

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Fixed cells were lysed and histone-DNA complexes were isolated with antibodies. Immunostaining half of the protocol includes cell dispensing, fixation, permeabilization, blocking, reaction to primary anti-body and the ChILT probe. The reactive probe are integrated into a proximal genomic region to where the primary antibody is bound upon Tn5 activation. The integrated ChILT DNA is used for the linear amplification of genomic sequences and the transcribed RNAs are utilized for library preparation for deep-sequencing. Fixed cells were lysed and histone-DNA complexes were isolated with antibodies. Libraries were prepared on the Illumina HiSeq1500 and 2500 platforms.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

mm10

Number of total reads
25682671
Reads aligned (%)
97.0
Duplicates removed (%)
11.1
Number of peaks
388 (qval < 1E-05)

mm9

Number of total reads
25682671
Reads aligned (%)
96.9
Duplicates removed (%)
11.2
Number of peaks
411 (qval < 1E-05)

Base call quality data from DBCLS SRA