GSM3151944: HEK293T.Cascade IL1RN crRNA 26.ctrl.rep3; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Kidney
Cell type
293
Primary Tissue
Kidney
Tissue Diagnosis
Normal
Attributes by original data submitter
Sample
source_name
HEK293T Cells
cell line
HEK293T
tissue
transformed human embryonic kidney cells
chip antibody
none
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were fixed with formaldehyde and lysed to isolate nuclei. Chromatin was sonicated and immunoprecipitated using an anti-FLAG antibody (M2, Sigma F3165). Libraries were prepared using the Hyper Prep kit (Kapa Biosystems) and samples were barcoded with Illumina Truseq indexes.