Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Kidney
Cell type
293
Primary Tissue
Kidney
Tissue Diagnosis
Normal

Attributes by original data submitter

Sample

source_name
HEK293T Cells
cell line
HEK293T
tissue
transformed human embryonic kidney cells
chip antibody
none

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were fixed with formaldehyde and lysed to isolate nuclei. Chromatin was sonicated and immunoprecipitated using an anti-FLAG antibody (M2, Sigma F3165). Libraries were prepared using the Hyper Prep kit (Kapa Biosystems) and samples were barcoded with Illumina Truseq indexes.

Sequencing Platform

instrument_model
Illumina HiSeq 4000

hg38

Number of total reads
32686798
Reads aligned (%)
98.3
Duplicates removed (%)
8.7
Number of peaks
912 (qval < 1E-05)

hg19

Number of total reads
32686798
Reads aligned (%)
97.7
Duplicates removed (%)
9.7
Number of peaks
1132 (qval < 1E-05)

Base call quality data from DBCLS SRA