Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K27me3

Cell type

Cell type Class
Embryo
Cell type
2-4h embryos
NA
NA

Attributes by original data submitter

Sample

source_name
2-4 hr old Oregon R embryos
devepmental stage
2-4 hr old embryo
antibodies
H3K27me3 (Cell Signaling (C36B11))

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Libraries were prepared according to Illumina's instructions accompanying the TruSeq kit.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

dm6

Number of total reads
18171780
Reads aligned (%)
94.4
Duplicates removed (%)
3.0
Number of peaks
2296 (qval < 1E-05)

dm3

Number of total reads
18171780
Reads aligned (%)
94.5
Duplicates removed (%)
2.9
Number of peaks
2561 (qval < 1E-05)

Base call quality data from DBCLS SRA