Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
pho

Cell type

Cell type Class
Embryo
Cell type
2-4h embryos
NA
NA

Attributes by original data submitter

Sample

source_name
2-4 hr old Oregon R embryos
devepmental stage
2-4 hr old embryo
antibodies
Pho (Papp and Müller 2006)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Libraries were prepared according to Illumina's instructions accompanying the TruSeq kit.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

dm3

Number of total reads
17458679
Reads aligned (%)
93.0
Duplicates removed (%)
5.1
Number of peaks
3799 (qval < 1E-05)

dm6

Number of total reads
17458679
Reads aligned (%)
92.4
Duplicates removed (%)
6.5
Number of peaks
3186 (qval < 1E-05)

Base call quality data from DBCLS SRA