Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
FOXP1

Cell type

Cell type Class
Blood
Cell type
B cells
NA
NA

Attributes by original data submitter

Sample

source_name
Germinal Center CD77+ B cells
tissue
Primary tonsilar B cells
chip antibody
FOXP1 (ab4729, Rabbit, 2 ug)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin was sheared to 200-400bp, incubated with H3K27ac antibody (abcam 4729) overnight at 4C on a rocker, antibody chromatin complex was captured by ProteinA agarose beads, washed with low salt, high salt, LiCl, and TE buffer, eluted and then de-crosslinked at 65C with 0.3M NaCl final for at least 6 hr. Final chormatin was purified using Qiagen PCR purification columns. Libraries were prepared using the Illumina Truseq ChIPseq Library preparation Kit following the manufacture's instructions (Illumina, CA).

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
205049278
Reads aligned (%)
60.7
Duplicates removed (%)
34.9
Number of peaks
15673 (qval < 1E-05)

hg38

Number of total reads
205049278
Reads aligned (%)
62.8
Duplicates removed (%)
33.3
Number of peaks
17385 (qval < 1E-05)

Base call quality data from DBCLS SRA