Antigen

Antigen Class

Histone

Antigen

H3K4me1

Cell type

Cell type Class

Prostate

Cell type

LNCAP

Primary Tissue

Prostate

Tissue Diagnosis

Carcinoma

Sample

source_name

cell line LNCaP

androgen_treatment

Y

hypoxia_treatment

Y

chip antibody

pAb 194-050

target protein

H3K4me1

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Genomic DNA was isolated using phenol:chloroform:isopropanol (25:24:1, Invitrogen), back-extracted with 200ul of TE, precipitated with isopropanol, washed with 75% ethanol, air-dried and resuspended in 60ul 10mM Tris-HCl pH 8. TruSeq ChIP kit from Illumina

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

32039625

Reads aligned (%)

97.5

Duplicates removed (%)

22.5

Number of peaks

20614 (qval < 1E-05)

hg19

Number of total reads

32039625

Reads aligned (%)

97.1

Duplicates removed (%)

23.1

Number of peaks

20692 (qval < 1E-05)