Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
AR

Cell type

Cell type Class
Prostate
Cell type
LNCAP
Primary Tissue
Prostate
Tissue Diagnosis
Carcinoma

Attributes by original data submitter

Sample

source_name
cell line LNCaP
androgen_treatment
Y
hypoxia_treatment
Y
chip antibody
AR N20
target protein
AR

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Genomic DNA was isolated using phenol:chloroform:isopropanol (25:24:1, Invitrogen), back-extracted with 200ul of TE, precipitated with isopropanol, washed with 75% ethanol, air-dried and resuspended in 60ul 10mM Tris-HCl pH 8. TruSeq ChIP kit from Illumina

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
30824336
Reads aligned (%)
82.5
Duplicates removed (%)
15.9
Number of peaks
41276 (qval < 1E-05)

hg38

Number of total reads
30824336
Reads aligned (%)
84.0
Duplicates removed (%)
14.9
Number of peaks
41311 (qval < 1E-05)

Base call quality data from DBCLS SRA