GSM3148400: ChIP-seq of HDA-1 in cfp-1, replicate 2; Caenorhabditis elegans; ChIP-Seq
Sample information curated by ChIP-Atlas
TFs and others
Cell type Class
Attributes by original data submitter
chip antibody supplier
Sequenced DNA Library
Chromatin immunoprecipitations and library preparations were conducted as in (McMurchy et al., 2017), using formaldehyde as a fixitive for the H3K4me3 ChIPs (30ug DNA, 2.5ug antibody) and formaldehyde and EGS as fixatives for the SIN-3 (15ug DNA, 2.5ug antibody) and HDA-1 (30ug DNA, 2.5ug antibody) ChIPs. Approximately 10% C. briggsae chromatin extract was spiked into the C. elegans extract for the H3K4me3 ChIPs and 5% into the HDA-1 ChIPs. The HDA-1 antibody did not detect C. briggsae HDA-1 and so was not used for normalization.