GSM3148394: ChIP-seq of SIN-3 in wild-type, replicate 2; Caenorhabditis elegans; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
sin-3
Cell type
Cell type Class
Embryo
Cell type
Mixed embryo
NA
NA
Attributes by original data submitter
Sample
source_name
Mixed Embryos
tissue
whole larvae
strain
N2
Stage
MxE
chip antibody
anti-SIN3
chip antibody supplier
Novus Biologicals
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin immunoprecipitations and library preparations were conducted as in (McMurchy et al., 2017), using formaldehyde as a fixitive for the H3K4me3 ChIPs (30ug DNA, 2.5ug antibody) and formaldehyde and EGS as fixatives for the SIN-3 (15ug DNA, 2.5ug antibody) and HDA-1 (30ug DNA, 2.5ug antibody) ChIPs. Approximately 10% C. briggsae chromatin extract was spiked into the C. elegans extract for the H3K4me3 ChIPs and 5% into the HDA-1 ChIPs. The HDA-1 antibody did not detect C. briggsae HDA-1 and so was not used for normalization.