Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Liver

Cell type

Liver

MeSH Description

A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.

Sample

source_name

Liver

strain

C57BL/6

experiment

ChIP-Seq

chip antibody

none

time point

36 h

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

ChIP-seq was performed with the 'MicroPlex Library Preparation' kit, from Diagenode 10 ng of immunoprecipitated material was used for sequencing library preparation. Total input was also prepared for sequencing at each time point and replicate. Paired-end sequencing libraries were prepared with the 'MicroPlex Library Preparation' kit, from Diagenode, following the instructions of the manufacturer (Diagenode, catalog no C05010011). No size selection was performed and the chromatin was amplified with 14 cycles of PCR. 100 nucleotides at the fragment ends were sequenced with paired-end sequencing technology from HiSeq 2500 (Illumina).

Sequencing Platform

instrument_model

Illumina HiSeq 2500

mm10

Number of total reads

52005026

Reads aligned (%)

94.3

Duplicates removed (%)

8.9

Number of peaks

254 (qval < 1E-05)

mm9

Number of total reads

52005026

Reads aligned (%)

94.1

Duplicates removed (%)

9.6

Number of peaks

262 (qval < 1E-05)