Antigen

Antigen Class

TFs and others

Antigen

EPAS1

Cell type

Cell type Class

Prostate

Cell type

PC-3

Primary Tissue

Prostate

Tissue Diagnosis

Adenocarcinoma

Sample

source_name

PC3 cells

protocol

Hypoxia

cell line

PC3 cells

tumor type

prostate cancer

antibody

HIF2A

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and protein-DNA complexes were isolated with antibody. ChIP-enriched DNA was used for library creation with the Rubicon ThruPLEX-FD kit and sequenced (75 bp single-end reads).

Sequencing Platform

instrument_model

NextSeq 500

hg19

Number of total reads

54556638

Reads aligned (%)

37.3

Duplicates removed (%)

56.2

Number of peaks

3870 (qval < 1E-05)

hg38

Number of total reads

54556638

Reads aligned (%)

38.3

Duplicates removed (%)

55.4

Number of peaks

3899 (qval < 1E-05)