Antigen

Antigen Class

Unclassified

Antigen

Unclassified

Cell type

Cell type Class

Epidermis

Cell type

A-375

Primary Tissue

Skin

Tissue Diagnosis

Melanoma

Sample

source_name

Melanoma cell line

cell line

A375

time point

Base line

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Chromatin was extracted using the MagnaChIP-seq kit (millipore). 4 µg of antibody was used on 2 million cells. Sonication was performed for 12 cycles, 30 sec pulses using the biorupter. Illumina TruSeq

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

48337505

Reads aligned (%)

89.1

Duplicates removed (%)

15.3

Number of peaks

25772 (qval < 1E-05)

hg19

Number of total reads

48337505

Reads aligned (%)

88.7

Duplicates removed (%)

16.0

Number of peaks

25673 (qval < 1E-05)