GSM3140973: Input Mnase Schlap1 replicate 1; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Prostate
Cell type
RWPE-1
Primary Tissue
Prostate
Tissue Diagnosis
Normal
Attributes by original data submitter
Sample
source_name
Benign Prostate Epithelial Cells (RWPE1)
cell line
RWPE1
tissue
Benign Prostate Epithelial Cells
antibody
N/A
input
N/A
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were fixed in 0.3% HCHO at 4 degrees for 30 minutes before quenching with 125mM glycine. For histone modifications chromatin was prepared by sonication. For SWI/SNF chromatin was processed by Mnase digestion before overnight immunoprecipitation with antibodies against targets of interest Libraries were created using Kapa hyperprep DNA library kit for Illumina sequencing