Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
SMARCA4

Cell type

Cell type Class
Prostate
Cell type
RWPE-1
Primary Tissue
Prostate
Tissue Diagnosis
Normal

Attributes by original data submitter

Sample

source_name
Benign Prostate Epithelial Cells (RWPE1)
cell line
RWPE1
tissue
Benign Prostate Epithelial Cells
antibody
Abcam ab110641
input
Mnase

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were fixed in 0.3% HCHO at 4 degrees for 30 minutes before quenching with 125mM glycine. For histone modifications chromatin was prepared by sonication. For SWI/SNF chromatin was processed by Mnase digestion before overnight immunoprecipitation with antibodies against targets of interest Libraries were created using Kapa hyperprep DNA library kit for Illumina sequencing

Sequencing Platform

instrument_model
Illumina HiSeq 4000

hg19

Number of total reads
13172736
Reads aligned (%)
99.8
Duplicates removed (%)
0.0
Number of peaks
101 (qval < 1E-05)

hg38

Number of total reads
6022144
Reads aligned (%)
100.0
Duplicates removed (%)
0.0
Number of peaks
53 (qval < 1E-05)

Base call quality data from DBCLS SRA