Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Embryo

Cell type

Embryonic basal ganglia

NA

NA

Sample

source_name

basal ganglia

strain

CD1

tissue

basal ganglia

developmental stage

Embryonic day 13.5

chip-antibody

none

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Tissue was micro-dissected, cross-linked and sheared to 200-1000bp fragment. Protein/DNA complexes were isolated with antibodies and collected using Dynabeads. ChIP-seq libraries were generated using the Ovation Ultralow DR Multiplex System (NuGEN #0330)

Sequencing Platform

instrument_model

Illumina HiSeq 4000

mm10

Number of total reads

30585381

Reads aligned (%)

97.8

Duplicates removed (%)

19.1

Number of peaks

394 (qval < 1E-05)

mm9

Number of total reads

30585381

Reads aligned (%)

97.6

Duplicates removed (%)

19.2

Number of peaks

420 (qval < 1E-05)