GSM3138016: T47D-TRPS1-rep2; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
TFs and others
Cell type Class
Attributes by original data submitter
TRPS1 ChIP antibody
Sequenced DNA Library
For the chip-seq: Libraries were prepared according to Illumina's instructions. Briefly, cells were crosslinked with 1% paraformaldehyde for 10 min at room temperature, quenched with glycine, and fixed chromatin was sonicated and immunoprecipitated with specific antibodies.RNA –seq: The total RNA was extracted with the RNeasy kit (Promega). For the chip-seq:Libraries were prepared with Illumina's ChIP-Seq sample prep kit for next-generation sequencing.For the RNA –seq: Followed by library preparation and next-generation sequencing.