GSM3138014: T47D-input-rep2; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Breast
Cell type
T-47D
Primary Tissue
Breast
Tissue Diagnosis
Adenocarcinoma Ductal
Attributes by original data submitter
Sample
source_name
input_ChIPseq
cell line
T47D
antibody
None
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
For the chip-seq: Libraries were prepared according to Illumina's instructions. Briefly, cells were crosslinked with 1% paraformaldehyde for 10 min at room temperature, quenched with glycine, and fixed chromatin was sonicated and immunoprecipitated with specific antibodies.RNA –seq: The total RNA was extracted with the RNeasy kit (Promega). For the chip-seq:Libraries were prepared with Illumina's ChIP-Seq sample prep kit for next-generation sequencing.For the RNA –seq: Followed by library preparation and next-generation sequencing.