Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
EHF

Cell type

Cell type Class
Prostate
Cell type
RWPE-1
Primary Tissue
Prostate
Tissue Diagnosis
Normal

Attributes by original data submitter

Sample

source_name
RWPE1-FLAG-EHF
cell line
RWPE1
cell type
normal prostate epithelial cells
infection
retrovirus expressing FLAG-EHF
chip antibody
anti-FLAG antibody

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and taggedprotein-DNA complexes were isolated with anti-FLAG antibody. Libraries were prepared for combined input for RWPE1-FLAG-ERG and RWPE1-FLAG-EHF, as well as ChIP DNA from FLAG immunoprecipitations form each cell line using NEBNext® ChIP-Seq Library Prep Master Mix Set for Illumina.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg19

Number of total reads
21737627
Reads aligned (%)
87.6
Duplicates removed (%)
5.5
Number of peaks
19028 (qval < 1E-05)

hg38

Number of total reads
21737627
Reads aligned (%)
89.7
Duplicates removed (%)
4.0
Number of peaks
19051 (qval < 1E-05)

Base call quality data from DBCLS SRA