GSM3138379: FLAG-ERG ChIPSeq; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
ERG
Cell type
Cell type Class
Prostate
Cell type
RWPE-1
Primary Tissue
Prostate
Tissue Diagnosis
Normal
Attributes by original data submitter
Sample
source_name
RWPE1-FLAG-ERG
cell line
RWPE1
cell type
normal prostate epithelial cells
infection
retrovirus expressing FLAG-ERG
chip antibody
anti-FLAG antibody
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and taggedprotein-DNA complexes were isolated with anti-FLAG antibody. Libraries were prepared for combined input for RWPE1-FLAG-ERG and RWPE1-FLAG-EHF, as well as ChIP DNA from FLAG immunoprecipitations form each cell line using NEBNext® ChIP-Seq Library Prep Master Mix Set for Illumina.