Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K27ac

Cell type

Cell type Class
Blood
Cell type
MOLM-13
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Acute Myelogenous

Attributes by original data submitter

Sample

source_name
Leukemic cell line MOLM-13 cells
cell line
MOLM-13
shRNA
sh-FIS1-#B
chip antibody
H3K27Ac (Active Motif Cat# 39133, RRID:AB_2561016)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
QIAGEN RNeasy Plus ThruPLEX DNA-seq Kit (Rubicon Genomics) Samples were barcoded and pooled in equimolar amounts and sent out for next-generation sequencing

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg38

Number of total reads
53610990
Reads aligned (%)
99.0
Duplicates removed (%)
35.0
Number of peaks
45348 (qval < 1E-05)

hg19

Number of total reads
53610990
Reads aligned (%)
98.6
Duplicates removed (%)
35.7
Number of peaks
45337 (qval < 1E-05)

Base call quality data from DBCLS SRA