Antigen

Antigen Class

Histone

Antigen

H3K27ac

Cell type

Cell type Class

Blood

Cell type

MOLM-13

Primary Tissue

Blood

Tissue Diagnosis

Leukemia Acute Myelogenous

Sample

source_name

Leukemic cell line MOLM-13 cells

cell line

MOLM-13

shRNA

sh-RNA Control

chip antibody

H3K27Ac (Active Motif Cat# 39133, RRID:AB_2561016)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

QIAGEN RNeasy Plus ThruPLEX DNA-seq Kit (Rubicon Genomics) Samples were barcoded and pooled in equimolar amounts and sent out for next-generation sequencing

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

53977169

Reads aligned (%)

98.9

Duplicates removed (%)

28.1

Number of peaks

36682 (qval < 1E-05)

hg19

Number of total reads

53977169

Reads aligned (%)

98.4

Duplicates removed (%)

29.1

Number of peaks

36809 (qval < 1E-05)