Antigen

Antigen Class

TFs and others

Antigen

GFP

Cell type

Cell type Class

Embryo

Cell type

7h embryos

NA

NA

Sample

source_name

Stage 11 embryos

chip antibody

rabbit anti-GFP antibody (1:100), Invitrogen A6455, Lot# 1603336

genotype

yan-/-(yanE833), Pbac{pnt-GFP.FPTB}VK00037

Stage

stage 11 embryo

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Hand-selected embryos were fixed with 1.8% formaldehyde. Lysates were clarified from sonicated nuclei andTF-DNA complexes isolated with an antibody. Libraries were prepared for sequencing using standard Illumina protocols.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

dm6

Number of total reads

57197110

Reads aligned (%)

39.1

Duplicates removed (%)

27.9

Number of peaks

8242 (qval < 1E-05)

dm3

Number of total reads

57197110

Reads aligned (%)

39.3

Duplicates removed (%)

26.4

Number of peaks

7666 (qval < 1E-05)