Antigen

Antigen Class

TFs and others

Antigen

gro

Cell type

Cell type Class

Embryo

Cell type

7h embryos

NA

NA

Sample

source_name

Stage 11 embryos

chip antibody

anti-Groucho antibody (1:200), Negre et al. (2011) Nature, 471:527-531

genotype

pnt -/- (pnt AF397)

Stage

stage 11 embryo

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Hand-selected embryos were fixed with 1.8% formaldehyde. Lysates were clarified from sonicated nuclei andTF-DNA complexes isolated with an antibody. Libraries were prepared for sequencing using standard Illumina protocols.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

dm6

Number of total reads

83805861

Reads aligned (%)

58.8

Duplicates removed (%)

27.2

Number of peaks

0 (qval < 1E-05)

dm3

Number of total reads

83805861

Reads aligned (%)

59.0

Duplicates removed (%)

25.4

Number of peaks

0 (qval < 1E-05)