Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
gro

Cell type

Cell type Class
Embryo
Cell type
7h embryos
NA
NA

Attributes by original data submitter

Sample

source_name
Stage 11 embryos
chip antibody
anti-Groucho antibody (1:200), Negre et al. (2011) Nature, 471:527-531
genotype
w1118
Stage
stage 11 embryo

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Hand-selected embryos were fixed with 1.8% formaldehyde. Lysates were clarified from sonicated nuclei andTF-DNA complexes isolated with an antibody. Libraries were prepared for sequencing using standard Illumina protocols.

Sequencing Platform

instrument_model
Illumina Genome Analyzer

dm3

Number of total reads
22145669
Reads aligned (%)
23.5
Duplicates removed (%)
57.8
Number of peaks
4904 (qval < 1E-05)

dm6

Number of total reads
22145669
Reads aligned (%)
22.5
Duplicates removed (%)
59.6
Number of peaks
5885 (qval < 1E-05)

Base call quality data from DBCLS SRA