anti-Groucho antibody (1:200), Negre et al. (2011) Nature, 471:527-531
stage 11 embryo
Sequenced DNA Library
Hand-selected embryos were fixed with 1.8% formaldehyde. Lysates were clarified from sonicated nuclei andTF-DNA complexes isolated with an antibody. Libraries were prepared for sequencing using standard Illumina protocols.