anti-Groucho antibody (1:200), Negre et al. (2011) Nature, 471:527-531
genotype
w1118
Stage
stage 11 embryo
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Hand-selected embryos were fixed with 1.8% formaldehyde. Lysates were clarified from sonicated nuclei andTF-DNA complexes isolated with an antibody. Libraries were prepared for sequencing using standard Illumina protocols.