Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
aop

Cell type

Cell type Class
Embryo
Cell type
7h embryos
NA
NA

Attributes by original data submitter

Sample

source_name
Stage 11 embryos
chip antibody
Guinea pig anti-Yan antibody (1:500), Webber et al. (2013) Genetics, 193:633-649
genotype
pnt -/- (pnt Delta88)
Stage
stage 11 embryo

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Hand-selected embryos were fixed with 1.8% formaldehyde. Lysates were clarified from sonicated nuclei andTF-DNA complexes isolated with an antibody. Libraries were prepared for sequencing using standard Illumina protocols.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

dm3

Number of total reads
96672516
Reads aligned (%)
29.9
Duplicates removed (%)
64.2
Number of peaks
0 (qval < 1E-05)

dm6

Number of total reads
96672516
Reads aligned (%)
25.0
Duplicates removed (%)
69.7
Number of peaks
11573 (qval < 1E-05)

Base call quality data from DBCLS SRA