Antigen

Antigen Class

TFs and others

Antigen

aop

Cell type

Cell type Class

Embryo

Cell type

7h embryos

NA

NA

Sample

source_name

Stage 11 embryos

chip antibody

Guinea pig anti-Yan antibody (1:500), Webber et al. (2013) Genetics, 193:633-649

genotype

pnt -/- (pnt Delta88)

Stage

stage 11 embryo

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Hand-selected embryos were fixed with 1.8% formaldehyde. Lysates were clarified from sonicated nuclei andTF-DNA complexes isolated with an antibody. Libraries were prepared for sequencing using standard Illumina protocols.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

dm3

Number of total reads

96672516

Reads aligned (%)

29.9

Duplicates removed (%)

64.2

Number of peaks

0 (qval < 1E-05)

dm6

Number of total reads

96672516

Reads aligned (%)

25.0

Duplicates removed (%)

69.7

Number of peaks

11573 (qval < 1E-05)