Antigen

Antigen Class

Histone

Antigen

H3K27me3

Cell type

Cell type Class

Blood

Cell type

Erythroblasts

MeSH Description

Immature, nucleated ERYTHROCYTES occupying the stage of ERYTHROPOIESIS that follows formation of ERYTHROID PRECURSOR CELLS and precedes formation of RETICULOCYTES. The normal series is called normoblasts. Cells called MEGALOBLASTS are a pathologic series of erythroblasts.

Sample

source_name

erythroblast

cell type

Adult erythroblasts

chip antibody

H3K27me3 (07-449) from Millipore

number of sonications

1

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

as described by Pope SH et al, Eur J Haematol 64:292-303, 2000 library construction was provided by Oxford Genomics Centre, or by Edinburgh Genomics

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

81554229

Reads aligned (%)

92.2

Duplicates removed (%)

46.2

Number of peaks

3240 (qval < 1E-05)

hg19

Number of total reads

81554229

Reads aligned (%)

91.8

Duplicates removed (%)

46.5

Number of peaks

2891 (qval < 1E-05)